Measurement

Part:BBa_M12072

Designed by: Will Whitney   Group: MIT BE 20.20 - S09 MIT BE 20.20 - S10   (2010-05-12)


CueR internal copper sensor

CueR is a copper-sensitive activator/repressor found in Yersinia pseudotuberculosis which performs a task for copper similar to that performed by merR for mercury. In absence of copper, it represses the transcription of the genes downstream of it (in source, cueTPAD), but when bound to copper it causes underwinding of cueOP DNA and thus promotes RNA polymerase transcription of the region. In the source genome, the following sequences are copper coping mechanisms.

Any genes inserted downstream of cueR will be transcribed only in the presence of copper inside the cell. Usable in E. coli and various other bacteria.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 28
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 133


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Categories
Parameters
None